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  • Ewing Sarcoma

    Methods and Protocols

    Series series Springer Protocols
    This second edition volume aims at providing new and updated methods detailing the most effective and widely employed protocols used in the Ewing sarcoma field. Chapters guide the readers through molecular biology techniques used to analyze Ewing sarcoma including tissue preservation and methods used in morpho-molecular diagnostics and routine immune-histological analysis. Additional wet-lab ... Leer más

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    Methods and Protocols

    Edición de Sweta Rani, Lukasz Skalniak ...
    Series series Springer Protocols
    This open access volume describes the computational and experimental models that help researchers understand the responses of the immune system in cancer and test experimental immune-oncology approaches. The chapters in this book cover methods such as profile polarization in macrophages using ELISA; immune checkpoint inhibitors; interactions of PD-1/PD-L1 and T cells; immunocompetent preclinical ... Leer más

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  • The Design and Development of Novel Drugs and Vaccines

    Principles and Protocols

    The Design and Development of Novel Drugs and Vaccines: Principles and Protocols presents both in silico methods and experimental protocols for vaccine and drug design and development, critically reviewing the most current research and emphasizing approaches and technologies that accelerate and lower the cost of product development. Sections review the technologies and approaches used to identify, ... Leer más

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  • Antimicrobial Peptides

    A Roadmap for Accelerating Discovery and Development

    Antimicrobial Peptides: A Roadmap for Accelerating Discovery and Development covers the most important efforts of scientists and engineers worldwide to accelerate the process of discovery, production, and eventual market penetration of more potent antimicrobial peptides. These efforts have been fueled by emerging technologies such as artificial intelligence and data science, molecular and CFD ... Leer más

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  • Tertiary Lymphoid Structures

    Methods and Protocols

    Edición de Marie-Caroline Dieu-Nosjean ...
    Series series Springer Protocols
    This second edition details new and updated chapters on technologies related to tertiary lymphoid structures( TLS). Chapters guide readers through TLS in cancers, different stages of TLS development, immune function of TLS, molecular, cellular, and imaging methods, and preclinical methods to uncover the function of cells composing TLS in inflammatory situations in humans. Written in the highly ... Leer más

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  • B-Cell Receptor Signaling

    Methods and Protocols

    Edición de Palash Chandra Maity ...
    Series series Springer Protocols
    B-lymphocytes produce antibodies, mediate humoral responses, and act as a key to the adaptive immune system of our body. The development and function of B cells are largely dependent on B cell antigen receptors (BCRs), which transmit a multitude of signals upon binding to the cognate antigen and activate the survival, proliferation, and differentiation of B cells thereof. Thus, studying the BCR ... Leer más

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  • Circulating Tumor Cells

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    Edición de Mark Jesus Magbanua ...
    Series series Springer Protocols
    This second editions explores new and updated approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Chapters detail comprehensive protocols for the capture of CTCs via filtration, density gradient centrifugation, microfluidic, immunomagnetic separation of CTCs, detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, flow ... Leer más

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  • Immunoproteomics

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    Edición de Kelly M. Fulton, Susan M. Twine ...
    Series series Springer Protocols
    This third edition volume expands on the previous editions with new chapters discussing advancements in mass spectrometry resolution, sensitivity, and speed, including the refinement of the identification of peptides presented on the surface of major histocompatibility proteins. These technologies continue to advance vaccine development, as well as cancer immunotherapy and precision medicine. ... Leer más

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  • Antibody Engineering

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    Edición de Damien Nevoltris, Romain Ollier ...
    Series series Springer Protocols
    This fourth edition volume expands on the previous editions by providing readers with a comprehensive guide to the latest foundational and advanced techniques used by researchers in the field. This book covers the construction of diverse antibody libraries including naïve, synthetic, and immune types and candidate selection using phage, yeast, or mammalian display systems. This book also discusses ... Leer más

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  • Regulatory T-Cells

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    Edición de Masahiro Ono ...
    Series series Springer Protocols
    This volume provides methods and models for investigating the immunosuppressive subset of CD4+ T-cells, regulatory T-cells (Treg). Chapters guide readers through, basic protocols for analyzing Treg in mice and humans, single-cell analysis for analyzing Treg, and methods for the analysis of Treg in clinical application. Written in the format of the highly successful Methods in Molecular Biology ... Leer más

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  • Cancer Immunosurveillance

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    Series series Springer Protocols
    This new edition explores the innovation and improvement of experimental techniques harnessed to dissect the complex relationship between cancer cells and the immune system of the host. Beginning with spectral and multicolor flow cytometric methods to characterize the immune contexture of different forms of metastases, the book continues with functional assays to study the activation status of ... Leer más

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  • Cell Death Part C

    Series Libro 208 - Methods in Cell Biology
    Cell Death: Part C, Volume 208 continues the comprehensive exploration of cellular mechanisms governing the fate of cells in health and disease. This volume delves deep into the molecular pathways, regulatory networks, and biological consequences of various cell death modalities, including apoptosis, necrosis, autophagy, ferroptosis, pyroptosis, and other emerging forms. - Explores advanced ... Leer más

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