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  • Photorespiration

    Methods and Protocols

    Edición de Berkley J Walker ...
    Series series Springer Protocols
    This volume provides methods on researching photorespiration to provide a better understanding of this vital pathway in plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting ... Leer más

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  • Mass Spectrometry-Based Proteomics

    Edición de Kris Gevaert ...
    Series series Springer Protocols
    This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by ... Leer más

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  • Protein Cages

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    Edición de Takafumi Ueno, Sierin Lim, Kelin Xia ...
    Series series Springer Protocols
    This volume provides the latest methods for synthesis, structural analysis, and elucidation of the mechanism. Chapters guide readers through methods on protein cages for nanotechnology, analyze designed protein cages, determine the structures, and even perform theoretical analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an ... Leer más

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  • Phytochromes

    Methods and Protocols

    Edición de Andreas Winkler ...
    Series series Springer Protocols
    This volume summarizes the state-of-the-art methodology for the functional characterization of phytochromes in vitro and in vivo. Chapters and divided into four parts covering, phytochrome discovery, spectroscopic techniques, x-ray crystallography, x-ray crystallography, and analyzing phytochrome function in vitro and *in vivo.*Written in the highly successful Methods in Molecular Biology series ... Leer más

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  • Fluorescent Proteins

    Methods and Protocols

    Edición de Mayank Sharma ...
    Series series Springer Protocols
    This volume brings together cutting-edge laboratory protocols to characterize the novel fluorescent proteins (FPs) and approaches based on fluorescent proteins that aim to answer some of the key cell biological questions. The book covers topics ranging from the database of fluorescent proteins to their characterization and adaptation to a wide range of biological systems. Written for the highly ... Leer más

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  • Protein Aggregation

    Methods and Protocols

    Edición de Andrzej Stanisław Cieplak ...
    Series series Springer Protocols
    The volume details techniques, methods, and conceptual developments to further the study of protein aggregation with emphasis on the pleiomorphic proteins implicated in etiology of neurodegeneration. Chapters guide readers through in vitro and in vivo studies of fibrillization and liquid-liquid phase separation processes, and offer a comprehensive account of the state-of-art of structural studies ... Leer más

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  • Oxygen Sensing

    Methods and Protocols

    Edición de Emily E. Weinert ...
    Series series Springer Protocols
    This volume provides researchers with protocols that help them investigate known or putative O2 sensing proteins and pathways. The chapters in this book discuss techniques ranging from anaerobic redox midpoint measurement to approaches to control expression of globin genes, which provide detailed methods for researchers interested in expanding their knowledge of O2 sensing systems. Written in the ... Leer más

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  • Molecular Motors

    Methods and Protocols

    Series series Springer Protocols
    This fully updated volume presents a diverse collection of protocols and methodologies for studying various molecular motors. The book explores the dynamics of the bacterial flagellar motor, the mechanisms of ATP synthase, the assembly and function of cytoskeletal motors, and the role of nucleic acid motors in cellular processes, while placing a strong emphasis on state-of-the-art techniques, ... Leer más

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  • Artificial Metalloproteins

    Series Libro 720 - Methods in Enzymology
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    Series series Springer Protocols
    This volume explores the latest integrated bioprocesses and technologies used to study the production of the target recombinant protein of therapeutic or diagnostic interest; its isolation, purification, and stabilization; and the bio-interaction and structural analyses. The chapters in this book are organized into four parts. Part One covers production methods of soluble and membrane proteins in ... Leer más

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  • Iron Metabolism

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    Edición de Oleh Khalimonchuk ...
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    This detailed volume explores classical and cutting-edge methods optimized and validated to analyze various aspects of iron metabolism, from in vitro to multi-organ level. Opening with a section on basic iron metabolism methods, the book continues with methods applicable to a variety of systems, ranging from bacteria to cultured mammalian cells and tissues, with a focus on cellular heme and iron ... Leer más

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